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piclv  (InvivoGen)


Bioz Verified Symbol InvivoGen is a verified supplier
Bioz Manufacturer Symbol InvivoGen manufactures this product  
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    InvivoGen piclv
    Piclv, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/poly+i+c+hmw+lyovec/Poly(I%3AC)+(HMW)+LyoVec/pmc13452298-28-6-4
    Average 95 stars, based on 68 article reviews
    piclv - by Bioz Stars, 2026-11
    95/100 stars

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    other:

    Article Title: Pharmacological targeting of RIG-I can selectively activate the integrated stress response.
    Article Snippet: MATERIALS AND METHODS Chemicals, antibodies, and reagents Thapsigargin (Thermo Fisher Scientific), ISRIB (Cayman Chemical), BtdCPU (MedChemExpress), CCCP (Cayman Chemical), 5′pppdsRNA (InvivoGEN), pyocyanin (Enzo Life Sciences), RIG012 (Axon Medchem), Orlistat (MedChemExpress), and CAY10499 (Cayman Chemical), poly(I:C)(HMW)Lyovec (InvivoGen) were purchased from the indicated companies.

    Article Title: Nef stabilizes actin to prevent HIV-1 sensing by RIG-I-like receptors.
    Article Snippet: PCR products were cloned into a linearized vector using the InFusion HD cloning kit (Takara, Cat#102518).

    Article Title: Pharmacological targeting of RIG-I can selectively activate the integrated stress response
    Article Snippet: Thapsigargin (Thermo Fisher Scientific), ISRIB (Cayman Chemical), BtdCPU (MedChemExpress), CCCP (Cayman Chemical), 5′ppp-dsRNA (InvivoGEN), pyocyanin (Enzo Life Sciences), RIG012 (Axon Medchem), Orlistat (MedChemExpress), and CAY10499 (Cayman Chemical), poly(I:C)(HMW)Lyovec (InvivoGen) were purchased from the indicated companies.

    Article Title: Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities.
    Article Snippet: Chemicals were obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.), Tokyo Chemical Industry (Tokyo, Japan), Acros Organics (Geel, Belgium), Enamine (Monmouth Junction, NJ, U.S.A.), and Apollo (Stockport, U.K.) and were used without further purification.

    Transfection:

    Article Title: Nef stabilizes actin to prevent HIV-1 sensing by RIG-I-like receptors
    Article Snippet: .. The following day, they were transfected with poly (I:C) HMW/lyovec (1 μg/mL, 200, 40 ng/mL) or G3-YSD/lyovec (1 μg/mL, 200, 40 ng/mL), according to the manufacturer’s protocol (Invivogen). .. PCR products were cloned into a linearized vector using the InFusion HD cloning kit (Takara, Cat#102518).

    Incubation:

    Article Title: Differential immunophenotype of circulating monocytes from pregnant women in response to viral ligands
    Article Snippet: .. For viral ligand stimulation, PBMCs were individually incubated with 2.5 μg/mL R848 (TLR7/8-based adjuvant; Cat# vac-r848; InvivoGen, San Diego, CA, USA), 1 μM Gardiquimod (TLR7 ligand; Cat# tlrl-gdqs; InvivoGen), 10 μg/mL Poly(I:C) (HMW) VacciGrade (TLR3-based adjuvant; Cat# vac-pic; InvivoGen), 50 μg/mL Poly(I:C) (HMW) LyoVec (RIG-I/MDA-5 ligand; Cat# tlrl-piclv; InvivoGen), and 2 μg/mL ODN 2216 (TLR9 ligand; Cat# tlrl-2216; InvivoGen) at 37 °C with 5% CO 2 for 24 h with the addition of protein transport inhibitor cocktail (Cat# 00-4980-03; ThermoFisher Scientific) for the last 4 h of incubation. ..

    Adjuvant:

    Article Title: Differential immunophenotype of circulating monocytes from pregnant women in response to viral ligands
    Article Snippet: .. For viral ligand stimulation, PBMCs were individually incubated with 2.5 μg/mL R848 (TLR7/8-based adjuvant; Cat# vac-r848; InvivoGen, San Diego, CA, USA), 1 μM Gardiquimod (TLR7 ligand; Cat# tlrl-gdqs; InvivoGen), 10 μg/mL Poly(I:C) (HMW) VacciGrade (TLR3-based adjuvant; Cat# vac-pic; InvivoGen), 50 μg/mL Poly(I:C) (HMW) LyoVec (RIG-I/MDA-5 ligand; Cat# tlrl-piclv; InvivoGen), and 2 μg/mL ODN 2216 (TLR9 ligand; Cat# tlrl-2216; InvivoGen) at 37 °C with 5% CO 2 for 24 h with the addition of protein transport inhibitor cocktail (Cat# 00-4980-03; ThermoFisher Scientific) for the last 4 h of incubation. ..



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    Image Search Results


    Microglia phagocytic activity and antigen cross presentation triggered by treatment with mock, poly(I:C), recombinant IFN γ , and PVSRIPO. The phagocytosis/cross presentation assay template with microglia exposure to B16 OVA , described in , was repeated with juxtaposing PVSRIPO to mock, (transfected) poly(I:C), and recombinant IFNγ. Phagocytosis of CD11b + -bead-isolated, hCD155 -tg mouse microglia was determined by flow cytometry analyses of CT-label (A , C) ; antigen cross presentation was tested by flow cytometry for H2Kb:SIINFEKL complexes (B , C) ; cells were tested 0h, 24h, 48h and 72h post treatment; flow cytometry histograms for phagocytosis/cross presentation at 48h and 72h post treatment are shown (C) . (A , B) Data were analyzed by Shapiro-Wilk test and 2-way ANOVA with multiple comparisons.

    Journal: bioRxiv

    Article Title: Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris

    doi: 10.64898/2026.04.06.716590

    Figure Lengend Snippet: Microglia phagocytic activity and antigen cross presentation triggered by treatment with mock, poly(I:C), recombinant IFN γ , and PVSRIPO. The phagocytosis/cross presentation assay template with microglia exposure to B16 OVA , described in , was repeated with juxtaposing PVSRIPO to mock, (transfected) poly(I:C), and recombinant IFNγ. Phagocytosis of CD11b + -bead-isolated, hCD155 -tg mouse microglia was determined by flow cytometry analyses of CT-label (A , C) ; antigen cross presentation was tested by flow cytometry for H2Kb:SIINFEKL complexes (B , C) ; cells were tested 0h, 24h, 48h and 72h post treatment; flow cytometry histograms for phagocytosis/cross presentation at 48h and 72h post treatment are shown (C) . (A , B) Data were analyzed by Shapiro-Wilk test and 2-way ANOVA with multiple comparisons.

    Article Snippet: Microglia were plated at 70% confluency and infected with PVSRIPO (MOI 10) or mock (DMEM), recombinant mouse IFNγ (200 U/ml; R&D Systems, #485-MI), or poly(I:C) (10 μg/ml; Invivogen, high molecular weight polyinosine:polycytidylic acid; LyoVec TM complexed, #tlrl-piclv) and incubated with 200,000 UV-irradiated cells for the duration of the intervals shown in the figures.

    Techniques: Activity Assay, Recombinant, Transfection, Isolation, Flow Cytometry